ALV
The ALV-Ag (p27) ELISA kit will measure the amount of antigen in various different samples taken from chickens. Microtitre plates have been pre-coated with anti-p27 antibody. Test samples are added to the microtitre wells where any p27 antigen present will bind and form an antigen-antibody complex. Non-specific antigens and other serum proteins are then washed away. Anti-p27 antibody labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any p27 antigen originally bound to antibody. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if p27 antigen is present and the intensity is directly related to the amount of p27 antigen present in the sample.
