Avian Reovirus Antibody
The REO ELISA kit will measure the amount of antibody to REO in the serum of chickens. Microtitre plates have been pre-coated with inactivated REO antigen. Chicken serum samples are diluted and added to the microtitre wells where any anti-REO antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti- REO antibodies originally bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti- REO antibody is present and the intensity is directly related to the amount of anti- REO present in the sample.
