Anigen Rapid CPV Ab Test Kit 2.0
Explanation of the Test:
Canine parvovirus (CPV) is the most important cause of viral gastroenteritits in dogs world-wide. Antibody titers to CPV are important in the prediction of canine health and preparing vaccination programs. The hemagglutination-inhibition (HI) test has been the most commonly used serological assay for CPV antibody.
Intended Use:
Anigen Rapid CPV Ab Test Kit 2.0 is a solid phase immunochromatographic assay for the rapid, quantitative detection of IgG antibodies to parvovirus in canine serum, plasma or whole blood. This test is intended for professional use as an aid in presumptive diagnosis, and preparation of vaccination programs. This test provides only a preliminary test result. Therefore, other tests like hemagglutination-inhibition test or serum neutralization test, or more specific alternative diagnosis methods must be used in order to obtain a confirmation of immune status.
Principle:
Anigen Rapid Ab Test Kit 2.0 is designed to detect IgG antibodies to CPV in serum, plasma or whole blood. Anigen Rapid CPV Ab Test Kit 2.0 has a letter of “T” as test line and “C” as control line on the surface of the device. The test line and the control line in result window are not visible before applying any
samples. The “C” line is used for procedural control. Control line should always appear if the test procedure is performed properly and the test reagents of the control line is working. A purple “T” line will be visible in the result window if there are IgG antibodies to CPV in the sample. If IgG antibodies to CPV are not present in the sample, then no color appears in the “T” line. When a specimen is added to the test, anti-CPV IgG in the specimen sample reacts with purified CPV antigen, and then reacts with monoclonal anti-CPV (conjugated gold) and forms a complex of antibodies and colloidal gold conjugates. As this mixture migrates along the length of the test kit by capillary action, the anti-CPV IgG complex is captured by the relevant anti-canine IgG, immobilized in the test line across the test kit and generates a colored line.
Materials provided(10Tests/Kit):
1) Ten(10) Anigen Rapid CPV Ab Test 2.0 Devices
2) Ten(10) Bottle containing 1ml of assay diluents
3) Ten(10) Disposable capillary tubes for specimen
4) Ten(10) Anticoagulant bottles
5) Ten(10) Disposable droppers
6) One(1) Color scale (1~6) measurement
7) One(1) Instruction for use
Precautions:
1) For best results, strict adherence to these instructions is required.
2) All specimens should be handled as being potentially infectious.
3) Do not open or remove test kits from their individually sealed pouches until immediately before their use.
4) Do not reuse test kit.
5) All reagents must be at room temperature before running the assay.
6) Do not use reagents beyond the stated expiration date marked on the package label.
7) The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
8) The assay diluents contains low concentration of sodium azide as a preservative. Sodium azide is toxic and should be handled carefully to avoid ingestion and skin contact.
Storage and Stability:
The kit can be stored at room temperature (2 ~ 30°C) or refrigerated. The test kit is stable through the expiration date marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.
Specimen Collection and Preparation:
1) Serum, plasma or whole blood samples should be used for this test.
2) Handle all blood products as capable of transmitting infectious diseases.
3) Whole blood samples should be used immediately, if possible or should be stored at 2 ~ 8°C up to 24 hours.
4) If serum or plasma specimens cannot be tested immediately, they should be refrigerated at 2 ~ 8C up to 2 weeks. Freezed specimen at –20C or below is available up to 1 year
5) Specimens containing precipitate may yield inconsistent test results. Such specimens must be clarified prior to assaying.
6) The use of hemolytic, lipaemic, icteric or bacterially contaminated specimens should be avoided. Erroneous result may occur.
Limitation of the Test:
1) This Test is for in-vitro diagnostic use only.
2) This test detects the presence of antibodies to CPV in the specimen and should not be used as the sole criterion for the diagnosis of CPV infection.
3) As with all diagnostic tests, all results must be considered with other clinical information available to the veterinarian.
4) For more accuracy of immune status, additional follow-up testing using other laboratory methods is recommended.
