Anigen Rapid FeLV Ag Test Kit
Explanation of the Test
Feline Leukemia Virus (FeLV) is a retrovirus that can directly cause cancer. The virus is usually transmitted through contact with the saliva of an infected cat. Kittens under 16 weeks are the most susceptible to the virus. Cats over 16 weeks who are dealing with an illness unrelated to FeLV, stress, or injury may also be more susceptible. Diseases caused by FeLV include lympho-sarcoma, myelogenous leukemia, thymic atrophy, nonregenerative anemia and panlukopenia-like disease. Because FeLV is immunosuppressive, it predisposes infected cats to variety of econdary diseases. The FeLV is excreted in saliva and tears and possibly the urine and feces of infected cats. Prolonged, extensive cat-to-cat contact is required for efficient spread, because the virus is rapidly inactivated by warmth and drying. The Anigen Rapid FeLV Ag Test Kit is a chromatographic immunoassay for the qualitative detection of FeLV antigen in feline whole blood, plasma or serum. The Anigen Rapid FeLV Ag Test Kit has the letters of T and C as “Test Line” and “Control Line” on the surface of the kit. Both the “Test Line” and “Control Line” in the result window are not visible before applying any samples. The “Control Lines” is used for procedural control and should always appear if this procedure is performed properly and the test reagents of the control line are working. A purple “Test Line” will be visible in the result window if there is enough FeLV antigens in the specimen. The specially selected FeLV antibodies are used in the test and as both capture and detector materials. These enable the Anigen Rapid FeLV Ag Test Kit to identify to FeLV antigen in specimens, with a high degree of accuracy.
Materials Provided
Anigen Rapid FIV Ab Test Kit contains following items to perform the assay
1) Ten(10) Anigen Rapid FeLV Ag Tests
2) One (1) Assay Diluent bottle
3) Ten (10) Disposable Capillary tube for specimens
4) Ten (10) Anticoagulant bottles
5) One (1) Instructions for use
A dark color score line on the capillary tube is the indicator line for 10㎕.
Storage and Stability
1) The Anigen Rapid FeLV Ag Test Kit should be stored at room temperature. The test device is sensitive to humidity and as well as to heat.
2) Perform the testing immediately after removing the test device from the foil pouch.
3) Do not use it beyond the expiration.
Test Procedure
1) Remove the test device from the foil pouch, and place it on a flat and dry surface.
2) Using the disposable capillary tube , add one (1) drop (approximately 10ul) of feline serum, plasma or whole blood into the sample hole, and then add two (2) drops (approximately 60ul) of the assay diluents.
3) As the test begins to work, you will see a purple color move across the result window in the center of the test device. If the migration has not appeared after 1 minute, add one more drop of the assay diluents to the sample well.
4) Interpret test results at 10 minutes. Do not interpret after 10 minutes.
Limitations of the Test
1) Anigen Rapid FeLV Ag Test Kit will only indicate the presence of FeLV in the specimen and should not be used as the sole criteria for the diagnosis of FeLV infection.
2) As with all diagnostic tests, all results must be interpreted together with other clinical information available to the veterinarian.
3) If the test result is negative and clinical symptom is persist, additional testing using other clinical methods is recommended. A negative result does not at any time preclude the possibility of FeLV infection.
