Se
The Se ELISA kit will measure the amount of antibody to S.e. and other group D salmonella species in the serum of chickens. Microtitre plates have been pre-coated with inactivated S.e. antigen. Chicken serum samples are diluted and added to the microtitre wells where any anti- S.e. antibodies present will bind and form an antigen-antibody complex. Non-specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti- S.e. antibodies originally bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti- S.e. antibody is present and the intensity is directly related to the amount of anti- S.e. present in the sample.
